Refine your DNA sequences with Twist Codon Optimization

Unlock the full potential of your DNA in seconds with our state-of-the-art codon optimization tool, built to maximize expression without compromising on manufacturability.
Optimize features other tools miss
Advanced algorithmsOur LLM-based optimization algorithm balances multiple variables across the sequence, not just codon usage. Reduce the risk of low protein expression seen with other methods.
Rapid expression-first optimizationWhere most algorithms make trade-offs in your sequence to improve manufacturability, ours doesn't need to. Optimize hundreds of sequences for expression in seconds.
Highly customizableStay in control of your optimization workflow. Select from over 150 species for expression, add flanking sequences, exclude restriction sites, or mask specific regions within sequences.
The optimization workflow, step by step
Input Your SequencesWhether you are working with one or hundreds of sequences, upload your sequences quickly with our ready-made template.
Set Your PreferencesSelect your host organism, restriction sites you want to avoid, and regions that you don't want to change.
OptimizeHit “optimize” and let us do the rest. You will be able to export your optimized sequences in seconds.
Implement & ExperimentImport your new sequences into our ordering portal, and start your synthesis project with genes optimized for expression.
A simple design tool that meets your needs
Simple, flexible sequence input
Drag and drop files or paste sequences directly into the table. Our interface supports bulk management and formatted templates to get your data structured and ready in seconds.
Precise host species selection
Select from a library of over 150 validated host species to ensure host-specific codon usage. The tool applies tailored biases to maximize potential protein yield while maintaining sequence stability.
Add flanks to your sequences
Add 5’ and 3’ flanking sequences to some or all of your sequences. New sites or blank fields will override any pre-existing flanking sites, giving you total control over your final design.
Update restriction sites
Add restriction enzyme cut sites to avoid or remove during optimization. You can also choose to only remove sites from your DNA sequence without further optimization to ensure downstream cloning compatibility.
Run optimization and download report
Execute your design in seconds and download a comprehensive optimization report. Review every sequence change and maintain a detailed technical record of your project before finalizing your order.
Generate ready to order sequences
Our engine provides sequences that are both optimized for expression and ready for synthesis. Instantly download a file formatted for our e-commerce checkout to go from design to order in one click.
*Performance may vary depending on sequence length, complexity, and system conditions.
For research use only. Not for use in diagnostic procedures.
FAQ
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Building genes encoding proteins?
Optimize them for expression in seconds with our state-of-the-art codon optimization app.
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